How to Select AAVs

Step 1: Determine which AAV serotype is best suited for your experiments.

There are more than 200 AAV serotypes and variants that have been discovered or created, and this number is constantly increasing. Each of these serotypes holds several unique features including differences in the ability of the virus to infect specific cell types or tissues – a characteristic referred to as tissue tropism. Since various AAV serotypes can differ substantially in cell type and tissue tropism, selection of an appropriate serotype is an important step in the experiment or therapeutic design process. For example, AAV DJ may often be the best choice for in vitro experiments due to its high infection rate across a broad range of cell lines, however, AAV9, AAVrh10 or AAVPHP.B may be better suited for in vivo experiments targeted at the central nervous system since these serotypes have been shown to penetrate the blood brain barrier.

At PackGene, we offer a wide range of commonly used AAV serotypes for packaging. Please review Table 1 and Table 2 below as a quick reference during the experiment or therapeutic design stage of your project. Commonly recommended serotypes are denotated with a red mark, however, our expert Technical Support team at PackGene would be happy to assist you in the selection of a serotype for your specific needs.

Table1. AAV for in vivo experiment

Step 2: Choose the vector fitting your experiments.

PackGene has developed a series of entry vectors that require only one-step cloning for rapid customization. Available entry vectors have been designed for a multitude of experimental approaches including:

• CRISPR-Knock-out (CRISPR-spCas9/saCas9/NmCas9/Cpf1)
• CRISPR-Activation
• Gene over-expression
• shRNA Knock-Down system
• miRNA-shRNA