Description
NLuc is a highly efficient bioluminescence system developed from a deep-sea shrimp luciferase. It offers superior light emission, stability, and versatility for various applications in mammalian cells. NanoLuc’s glow-type luminescence is significantly more potent than traditional luciferases, and it remains stable under harsh conditions. It can be tailored for high sensitivity, dynamic response, or export to culture medium without cell lysis. NanoLuc also enables luminescent assays for protein metabolism and localization within cells, even at low expression levels. This mRNA possesses a Cap1 structure, which is highly efficient in capping. It is fully substituted with N1-methyl-pseudo Uridine to improve expression and decrease immunogenicity. Additionally, the mRNA features a 110A tail in its sequence, resembling a mature mRNA.
| Gene name | Nluc |
|---|---|
| Function | Reporter |
| ORF sequence | ATGAACTCCTTCTCCACAAGCGCCTTCGGTCCAGTTGCCTTCTCCCTGGGCCTGCTCCTGGTGTTGCCTGCTGCCTTCCCTGCCCCAGTCTTCACACTCGAAGATTTCGTTGGGGACTGGCGACAGACAGCCGGCTACAACCTGGACCAAGTCCTTGAACAGGGAGGTGTGTCCAGTTTGTTTCAGAATCTCGGGGTGTCCGTAACTCCGATCCAAAGGATTGTCCTGAGCGGTGAAAATGGGCTGAAGATCGACATCCATGTCATCATCCCGTATGAAGGTCTGAGCGGCGACCAAATGGGCCAGATCGAAAAAATTTTTAAGGTGGTGTACCCTGTGGATGATCATCACTTTAAGGTGATCCTGCACTATGGCACACTGGTAATCGACGGGGTTACGCCGAACATGATCGACTATTTCGGACGGCCGTATGAAGGCATCGCCGTGTTCGACGGCAAAAAGATCACTGTAACAGGGACCCTGTGGAACGGCAACAAAATTATCGACGAGCGCCTGATCAACCCCGACGGCTCCCTGCTGTTCCGAGTAACCATCAACGGAGTGACCGGCTGGCGGCTGTGCGAACGCATTCTGGCGTAA |
| Species | human codon-optimized, Oplophorus gracilirostris |
| Cellular localization of the protein | Cytosol |
| Reference/PubMed link | |
| Concentration | 1mg/mL |
| Storage Buffer | Rnase Free H2O |
| Gene length (bp) | 600 |
| Molecular Weight (kDa) | 377 |
| Storage Temperature | -80°C |
| Shipping Condition | Dry ice |
| Appearance | Clear and colorless |
| Purity | Fragment analysis by capillary electrophoresis |
| Base Composition | N1meψ |
| Capping | Cap1 |
| Tag | N-HA |
| Stock | In stock |
