AAV Production Workflow:
Day1: Bacteria preparation
Day2: Plasmid preparation & enzyme digestion verification
Day5-7: Co-tranfection in Hek293T cells
Day8: Crude purification
Day9: Concentration and Ultracentrification
Day10: QC test (QPCR, endotoxinremoval, SDS-PAGE)
Day11: QA Review and Release
1μg plasmid from customer or made by PackGene → Bacterial transformation → Plasmid preparation → Co-transfection of 293T cells → Crude purification → Concentration → Ultra-centrifuge iodixanol density-gradient → 0.2μm sterile filter → qPCR titration → SDS-PAGE coomassie blue staining endotoxin testing → AAV certification AAV QC reports
Storage Requirements
- Store the virus at -80°C, take it out when needed, and place it on ice during operation. Fast Service must be used within 24 hours.
- Calculate the usage in advance, and PackGene will aliquot the virus according to the pre-determined requirements before shipping to avoid thawing and re-freezing after receiving the your AAVs as freeze thaw cycles influence virus viability. If aliquoting is required, it is recommended to use PCR tubes with siliconized inner walls, or special virus preservation tubes with low protein binding rates.
- Melt the virus in an ice bath and dilute with PBS or PBS / 0.001% F-68 if needed.
PackGene provides superior quality AAV packaging services to support your AAV-based programs. We have developed a series of proprietary technologies that greatly improve AAV production outcomes including titer, purity, potency, and consistency.
We offer multiple serotypes to meet your research needs, and strive to achieve rapid turnaround and affordable prices while maintaining the highest quality. We are committed to delivering AAVs at guaranteed quantities and within the quoted project lead time. In a case where our quantity and efficiency guarantee is not met, we will refund 5% of the total order cost as an account credit that may be applied to your next order.
