AAV Library Service

Advanced AAV Library Services
At PackGene, we offer a comprehensive range of AAV library services designed to advance your gene therapy research. Our expertise in AAV library creation and screening allows us to provide solutions tailored to your specific needs, enhancing gene delivery specificity and efficiency while minimizing off-target effects and bypassing innate immune responses.

Our capsid library services focus on screening novel AAV serotypes to optimize tissue specificity and serotype tropism. Using cutting-edge techniques like DNA shuffling, we generate diverse AAV serotypes that offer distinct advantages in gene therapy applications. This enables us to:

  • Enhance the specificity and efficiency of gene delivery
  • Minimize the risk of off-target effects
  • Bypass innate immune responses

 

Our Gene of Interest (GOI) library services involve promoter engineering to optimize gene expression and improve promoter tissue specificity. This ensures that your therapeutic genes are expressed at the right levels in the desired tissues, providing:

  • Optimized gene expression
  • Improved promoter tissue specificity

We provide comprehensive Gene editing library services for gene function screening, gene knockdown studies, disease modeling, cancer research, and drug target discovery. Our libraries include custom CRISPR sgRNA libraries, CRISPR activation libraries, CRISPRi libraries, CRISPR knockout libraries and shRNA libraries, enabling high-throughput screening to identify critical genes and pathways involved in various diseases and uncover potential therapeutic targets., facilitating:

  • Gene function screening
  • Disease modeling
  • Drug target discovery
  • Cancer research
Why Choose Us?
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Expertise in providing industrial leading AAV packaging service
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Innovative Techniques such as DNA shuffling, precision mutation or barcoding to handle various library design
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Diverse Library Designs

Random peptide Library

Up to 20 amino acids each site with the random peptide insertion Combinatorial mutation throughout the peptide insertion.

Precision Library

A short precision oligo pool that encodes gRNA library or other predesigned sequence insert into the vector. Each variant sequence can be predefined and precisely synthesized

DNA shuffling Library

Pre-synthesized gene library ligate together to create a combinatorial gene shuffling library.

~100 Capsid Genes ready to use

Barcoded Library

Your variant will be labeled with a barcode that adjacent or distant from the variable region. The barcode will enable easy variant identification by NGS or track cell lineage.

More library design we can handle

Site-Saturation Library

Up to 20 amino acids at single sites
1 amino acid change per variant

Saturation Scanning Library

Up to 20 amino acids at a series of sites you choose
1 amino acid change per variant

Combinatorial Mutant Library

You define the amino acid or nucleotide components and position; we generate the library from the template. Multiple mutation per variant in a combinatorial way

Library performance

Library quality
Near 100% coverage of our precision library
Excellent library uniformity
Low mispackaging rates
Mismatched capsid versus genome can be assessed by introducing a low frequency of capsid genes with early stop codons. The frequency of these stop codons indicates the mismatched rate. Typically, we achieve a mispackaging rate of less than 0.1% using our special AAV library packaging protocol, ensuring that NGS of the enriched capsid gene in the targeted tissue accurately represents the capsids specifically targeting that tissue.
Screening result
Final validation of novel muscle targeting Capsid screened in Cynomolgus Monkey
Resources
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Download ASGCT poster with 2024 data

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Download Application Note

Contact Us

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Related Services / Products

AAV Vector Design & Construction

End-to-end AAV vector design and construction solutions

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AAV Packaging Services

Ranging from pilot to industrial-scale AAV packaging for both in vitro and in vivo studies.

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Plasmid Preparation

Ideal for any phase of the drug development cycle

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