Lentivirus Plasmid Design and Construction

Gene Overexpression Vectors

• Gene Overexpression

  Our lentivirus vectors have been engineered to enhance gene expression within target cells, making them invaluable tools for studying gene function, modeling diseases, and exploring therapeutic applications. We clone your gene of interest into our pre-designed vectors, ensuring sustained expression through genomic integration.

  To access our Lentivirus over-expression cloning vectors and gene elements library, please check piVector Designer

  Available upon request, please contact our technical support team

  Vector ID	Promoter	GOI capacity	Promoter for Selection marker	Selection marker
  XL03C	CMV	3.3Kbp	EF1	CopGFP-2A-Puro
  XL10C	CMV	4Kbp	EF1	mRuby2
  XL11C	CMV	4Kbp	EF1	mCitrine
  XL12C	CMV	4Kbp	EF1	EYFP
  XL22C	CMV	3.2kp	EF1	mCherry-2A-Neo
  XL23C	EF1	4.2kb	T2A	CopGFP

• CRISPR-Based Gene Editing and Regulation

  PackGene offers high-quality lentivirus CRISPR vectors for precise gene editing and regulation. We offer plasmids for the expression of SpCas9, SaCas9, or gRNA, either separately or combined in an all-in-one vector. Our lentivirus Cas9 vector is de novo synthesized on a verified backbone, ensuring reliability and high production yields during lentivirus manufacturing.

  Design your lentivirus CRISPR vector via piVector Designer or contact our Ph.D.-level tech support team.

• shRNA Vectors

  PackGene’s lentivirus shRNA vectors are engineered for gene silencing by expressing shRNA sequences, that are processed into siRNA to target and degrade mRNA. These vectors are important tools for knocking down gene expression and observing phenotypic changes. Additionally, we offer lentivirus shRNA cloning for custom vector construction. To access our lentivirus shRNA vector library and design your vector, please check piVector Designer.

  Available upon request, please contact our technical support team to assist your design.

  Vector ID	Promoter	Promoter for Selection marker	Selection marker
  XL03B	U6 promoter	EF1a	EGFP-2A-Neo
  XL04B	U6 promoter	CMV	CopGFP-2A-Puro
  XL05B	U6 promoter	CMV	Puro
  XL06B	U6 promoter	PGK	Puro-lRES-mCherry
  XL07B	H1 promoter	CMV	CopGFP
  XL08B	H1 promoter	CMV	CopGFP-2A-Puro
  XL09B	H1 promoter	CMV	Puro
  XL012B	U6 promoter	CMV	Neo

• miRNA Vectors

  PackGene’s lentivirus miRNA vectors are customizable for overexpression or inhibition of specific miRNAs to provide insights into miRNA-mediated gene regulation. Lentivirus miRNA cloning is a valuable tool for research and developing miRNA-based therapies, particularly in cancer.

  Vector design and construction are available upon request, please contact our technical support team.

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