Lentivirus Packaging Services

PackGene offers end-to-end lentiviral vector design and packaging services, leveraging proprietary technologies and reagents for superior quality. Our optimized packaging enhances titer, purity, viability, and consistency, particularly for third-generation lentiviral vectors. Unlike plasmid DNA, which enables only transient expression, lentiviral vectors integrate transgenes into the host genome for stable expression across diverse cell types, including neurons, liver, heart, tumor, endothelial, and stem cells. Well-tolerated in mammalian systems, lentiviral vectors are suitable for both in vitro and in vivo applications.

Our 3rd-generation lentiviral systems feature a self-inactivating 3’ LTR, ensuring efficient infection of target cells without further viral replication. PackGene’s lentiviral products support the expression of coding and non-coding genes, including shRNA and microRNA.

Key Benefits

Efficient and prompt

delivery time as quick as 8 to 12 business days
Accurate titer measurements

post-transduction qPCR eliminates any overestimation concerns
Professional technical support

a response to any inquiries or requests will be delivered within 1 business day
Enhanced Safety

“Self-inactivating” 3rd-generation system increases biosafety
One-stop solution

from vector design and virus packaging to thorough analysis and testing
Secure shipping

Dry ice and cold chain logistics to maintain optimal efficacy

Service Details

	In vitro grade	In vivo grade
Application	Cell culture	In vivo study
Quantity	5E7 TU and up	5E7 TU and up
Functional titer (post-transduction qPCR)	≥1E+8TU/mL	≥1E+8TU/mL
Physical titer (p24 or qPCR)	≥ 2E+9 GC/mL ≥ 1E+10 LP/mL	≥ 2E+9 GC/mL ≥ 1E+10 LP/mL
Purification	Crude	Sucrose gradient
QC (see add-on QC below)	Post-transduction qPCR, fluorescence images if contain fluorescence	Post-transduction qPCR, fluorescence images if contain fluorescence
Timeline	Start from 7 business days	3-4 weeks

Lentivirus will be offer at a concentration of >1E8 TU/mL
Yield is not guaranteed for constructs with elements detrimental to the viral packaging process. This includes but is not limited to, toxic genes, genes that disrupt viral packaging, genes that disrupt cell or lentiviral RNA integrity, sequences prone to rearrangement or secondary structures, nucleoproteins, transmembrane proteins, receptor genes, and gene segments with over 6.5kb between LTRs.

Quality Control

Category	QC test	Description	Release standard
Titer	qPCR	qPCR quantifies the number of viral genome copies providing an accurate measure of the viral titer	Report titer
	Post-transduction  qPCR	Infection of cells followed by quantitative qPCR to determine the titer of lentiviruses	Report transduction unit
	Transduction test	Gradient dilution of infected cells and count of fluorescent cells	Provide bright field and fluorescent microscopy images
	p24 Elisa*	ELISA test for the p24 protein, a core capsid protein of HIV that is used to measure lentivirus titer	Report titer
Contamination	Mycoplasma test*	Measure mycoplasma DNA by PCR	Guaranteed negative
Contamination	Bioburden*	Plate count method: Quantification of live microorganisms (fungi, bacteria, etc.) using colony-forming units (CFUs)	No growth on the agarose plate
Safety	Endotoxin LAL*	Measure endotoxin level by gel clot assay	Report

* QC with additional charges

Need control lentivirus?

Off-the-shelf lentivirus	Virus per vial	Titer
CAG.EGFP.T2A.Puro.WPRE	2E7 TU	≥1E+8TU/mL
CAG.EGFP.T2A.Bsd.WPRE
CAG.EGFP.T2A.Hygro.WPRE
CAG.mCherry.T2A.Puro.WPRE
CAG.mCherry.T2A.Bsd.WPRE
CAG.mCherry.T2A.Hygro.WPRE
EF1.SpCas9.T2A.Puro.WPRE
EF1.SpCas9.T2A.EGFP.WPRE
U6.NCshRNA.CAG.EGFP.T2A.Puro.WPRE
U6.NCshRNA.CAG.mCherry.T2A.Bsd.WPRE

Performance

Post-Transduction Titer Ensure batch-to-batch consistency

No more concerns of overestimation
Accurate titer measurements by post-transduction qPCR

96-well plate, 1.5E+04 cells/well, virus 1.8µL, 72hrs after transduction See more QC test data

How we measure Post-Transduction Titer

qPCR: This test uses quantitative polymerase chain reaction (qPCR) to determine the amount of viral RNA present in cells after they have been transduced with lentivirus. This assay is used to determine the efficiency in which a lentivirus delivers its genetic material into host cells.

Post-transduction-qPCR-scaled

Transduction test by Fluorescent microscopy: In this test, a sample of cells are infected with the lentivirus, and transfection efficacy is determined by measuring either transgene expression or the expression of a reporter gene such as GFP (Green Fluorescent Protein). This assay is used to evaluate the efficiency with which the lentivirus drives transgene expression in target cells.

	Bright-field	Fluorescent field		Bright-field	Fluorescent field
96-well plate, 1.5E+04 cells/well, virus 18µL, 72hrs after transduction			96-well plate, 1.5E+04 cells/well, virus 1.8E-03µL, 72hrs after transduction
96-well plate, 1.5E+04 cells/well, virus 1.8µL, 72hrs after transduction			96-well plate, 1.5E+04 cells/well, virus 1.8E-04µL, 72hrs after transduction
96-well plate, 1.5E+04 cells/well, virus 1.8E-01µL, 72hrs after transduction			96-well plate, 1.5E+04 cells/well, virus 1.8E-05µL, 72hrs after transduction
96-well plate, 1.5E+04 cells/well, virus 1.8E-02µL, 72hrs after transduction			96-well plate, 1.5E+04 cells/well, virus 1.8E-06µL, 72hrs after transduction