Cap 1 N1meψ Cre-EGFP mRNA
Cre-Lox recombination is a highly versatile site-specific recombinase technology employed to perform deletions, insertions, translocations, and inversions at precise locations within cell DNA. Its adaptability allows for targeted DNA modifications tailored to specific cell types or triggered by external stimuli, utilized in both eukaryotic and prokaryotic systems. Particularly in neuroscience, Cre-lox recombination has facilitated the study of complex brain structures and neural circuits underlying cognition and behaviors. Widely utilized as an experimental tool, the Cre-loxP system enables conditional gene manipulation, offering spatial and temporal control for investigating genes of interest in tissue or cell-specific contexts. in this mRNA, Cre and EGFP genes are connected with P2A, thus the two reading frames will be translated into two proteins folding independently due to ribosome skipping This mRNA possesses a Cap1 structure, which is highly efficient in capping. It is fully substituted with N1-methyl-pseudo Uridine to improve expression and decrease immunogenicity. Additionally, the mRNA features a 110A tail in its sequence, resembling a mature mRNA.