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Cap 1 N1meψ EGFP mRNA
EGFP, originating from Aequorea victoria and introduced in 1996, is a fundamental green fluorescent protein. It is characterized as a rapidly maturing weak dimer with moderate sensitivity to acidic conditions. This mRNA possesses a Cap1 structure, which is highly eficient in capping. It is fully substituted with N1-methyl-pseudo Uridine to improve expression and decrease immunogenicity. Additionally, the mRNA features a 119A tail in its sequence, resembling a mature mRNA.
SKU: RNAP005
title
content
Gene name
EGFP
Function
Reporter
ORF sequence
ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTGA
Species
Aequorea victoria
Cellular localization of the protein
Cytosol
Reference/PubMed link
https://www.fpbase.org/reference/66/
Concentration
1mg/mL
Storage Buffer
Rnase Free H2O
Gene length (bp)
720
Molecular Weight (kDa)
380
Storage Temperature
-80°C
Shipping Condition
Dry ice
Appearance
Clear and colorless
Purity
Fragment analysis by capillary electrophoresis
Base Composition
N1meψ
Capping
Cap1
Tag
N-HA
Stock
In stock