The influx of calcium ions serves as an important intracellular signal across tissues and cell types. Calcium influx is a particularly important signal in neurons and is believed to act as a sufficient indicator of neuronal cell firing. By using special fluorescent dyes or calcium ion indicators such as GCaMP, we can determine real-time changes in calcium ion concentration for the purposes of measuring neuronal activity. GCaMP expression is commonly driven by AAV vectors and functions as a powerful and popular research tool.
PackGene provides a pre-made “off the shelf” AAV-GCaMP to drive the expression of GCaMP. These AAVs can be used directly, conveniently, and efficiently for in vivo experiments, and come standard with a complete quality inspection report.
GCaMP-AAV offers rapid and selective calcium concentration signaling in which fluorescence intensity correlates directly with calcium ion concentration. PackGene’s GCaMP-AAV reliabilityt is ensured with a complete quality inspection report. It can be readily applied in cell culture or in vivo experiments and thus offers both convenience and efficiency. Moreover, GCaMP can be targeted to specific cell subtypes by carefully selecting vector promoters and AAV serotypes to enhance both ersatility and applicability.
