We have multiple Entry plasmids and Pre-made AAVs portfolio may cover what you need.
Guided by short sgRNA (14-16nt) with modified scaffold, wild type SpCas9 will bind at the target site and won’t release, thus the MS2-P65-HSF1 component can bind with the modified loops in sgRNA scaffold and activate the nearby transcription, which possibly lead to more than 1000 folds augment of gene expression*.
|Vector#||Promoter||Activation componets||(Sp)sgRNA||Working with|
|E1||CMV||MS2-P65-HSF1||U6p-driven, w/MS2 loop||A1/A2/B1/B2/SpCas9 mouse|
|E2||CMV||MS2-P65-HSF1-2A-Cre||U6p-driven w/MS2 loop||A1/A2/B1/B2/SpCas9; floxed X-gene mouse|
* Nature Biotechnology 2015 33(11):1159-1161.
How these vectors play?
Customer will provide the sgRNA (14-16nt) target sequence for cloning. E1/E2 work with A1/A2/B1/B2/ SpCas9 mouse; E2 additionally expresses Cre are suitable for SpCas9 crossing GOI-floxed mouse.